RNAseq: Biological replicates vs sequencing depth

  • Posted on: 9 September 2016
  • By: vikram

If you are gearing up to do a RNAseq experiment and wondering if you should sequence deeper or sequence more biological replicates, this article - which I find still relevant - would be very useful.

RNA-seq differential expression studies: more sequence, or more replication?
Liu, Zhou and White 2013
http://bioinformatics.oxfordjournals.org/content/early/2013/12/06/bioinf...

Moreover, there is another source for getting some help designing an RNAseq experiment: Scotty.
http://scotty.genetics.utah.edu/

Finally, a comprehensive review of RNAseq methodology is here:
http://rnaseq.uoregon.edu/